Original Article

Effect of Pretreatment with Cilostazol on Spinal Cord Ischemia-reperfusion Injury in Rats

10.4274/eamr.galenos.2021.02411

  • Ülkü Kafa Kulaçoğlu
  • Yusuf Kalko
  • Gözde Erkanlı Şenturk

Received Date: 26.11.2020 Accepted Date: 03.04.2021 Eur Arc Med Res 0;0(0):0-0 [e-Pub]

Objective:

Following the aortic aneurysm repair surgery, ischemic spinal cord injury is a substantial pathologic outcome which may lead to paraplegia. This study aims the research of protective effect of cilostazol, which is a phosphodiesterase type-3 inhibitor, against ischemic/reperfusion-induced spinal cord injury that is experimentally forged in medulla spinalis of rats.

Methods:

A total of 24 rats were divided into 3 workgroups. the control group (n=8); the ischemia group (n=8), which aortic clamping applied without cilostazol administration ; and the cilostazol-adminsistered group (n=8). All animals were exposed to ischemia for 45 minutes by clamping the abdominal aorta. Afterwards, blood build up was provided by de-clamping. Serial assessments of motor and sensory functions of all rats were performed before the operation and, at 24 and 48 hours of reperfusion, using Tarlov scores and the score of LeMay. Spinal cords were harvested for histopathologic examination.

Results:

Tarlov scores at postoperative hours 24 and 48 tended to be higher in the cilostazol-treated group than in the nontreated ischemia group (3.13±0.64 versus 1.25±0.71, p=0.0029 for the 24th hour; 2.75±0.71 versus 0.38±0.52, p=0.0016 for the 48th hour). LeMay scores at postoperative hours 24 and 48 tended to be higher in the cilostazol-treated group than in the nontreated ischemia group (9.13±1.13 versus 4.50±0.76, p=0.0018 for the 24th hour; 9.00±1.20 versus 3.75±0.89, p=0.0018 for the 48th hour). Histologic outcomes correlated well with the neurologic outcomes.

Conclusion:

The results of this research have proven to us that pre-ischemia cilostazol treatment has a protective effect against ischemia/reperfusion-induced spinal cord injury.

Keywords: Ischemia/Reperfusion,Spinal Cord Injury,Cilostazol - Rat,Animal Model